The correlation between TEWETGQI (present in medial portion of ASP2, M-ASP2) immunodominant frequency and protection is reinforced by the result of C3H/He mice that were immunized using recombinant influenza encoding the C-terminus portion of ASP2 as prime and Ad-ASP2 as boost presented a survival curve similar to single immunized or homologous prime-boost using Ad-ASP2 after infection (p = 0.46). Here, BACE1 is linked to infection.