Thus, our observations indicate that a single immunization using Ad-ASP2 suffice to stimulate a significant production of effector cytokines IFN-γ, TNF-α and mobilize CD107a, elicit an immunodominant effector population which can control parasitemia, reduce tissue pathology and prolong survival when compared to control immunized mice even in a susceptible model. This evidence concerns the gene IFNG and parasitic infectious disease.