The high carrier rate, up to 18.2%, of the p.V37I variant from this selected cohort may justify genetic testing focusing upon the p.V37I variant of GJB2, when the pediatric subjects show moderate or slight hearing impairment without any sign of syndromic hearing impairments or any inner ear anomaly, and if they show sporadic or autosomal recessive hereditary patterns. This evidence concerns the gene GJB2 and hearing loss disorder.