ELISA and Western blot analysis revealed that VEGF, HGF and zonulin were present in higheramounts in glioma C6 CM than in astrocyte CM, whereas EGF and PGE2 were more abundant inastrocyte CM, in agreement with previous observations showing the expression of Zonulin in humangliomas [22], the lack of EGF and thesignificant production of HGF and VEGF in human glioma cell lines U251 and U87 [11]. The gene discussed is EGF; the disease is central nervous system cancer.