We have found that HGF, VEGF, zonulin and PGE2 inthe absence of EGF in glioma C6 CM induce transmigration, that VEGF, zonulin and PGE2open the BBB, that ReNcells CX express CRTAM, occludin and claudins 1, 3 and 4 that might facilitatetheir paracellular migration and that at the sites of transmigration the expression of occludin andclaudin-5 diminishes in RBMECs. The gene discussed is EGF; the disease is central nervous system cancer.