The reduction of the 14.2 kDa MBP isoform and the increase in the 12.4 kDa MBP fragment in EAE animals with stage 3 diseases is clearly evident in the SELDI mass spectra shown in Figure 3A. Proteolysis has long been thought to play a role in multiple sclerosis, and elevated proteolytic activity has been observed in cerebrospinal fluid in MS patients [62]. Here, MBP is linked to multiple sclerosis.