Furthermore, multivariate Cox regression analysis revealed that PNCK expression(p<0.001 ), N stage(p<0.001 ) and Fuhrman grade(p<0.001 ) were independent predictors for the overall survival of ccRCC patients (p<0.001, p<0.001, p<0.001; Table 2), whereas the other factors were not independently related to the survival of ccRCC patients. The gene discussed is PNCK; the disease is nonpapillary renal cell carcinoma.