C5AR2 and obesity due to melanocortin 4 receptor deficiency: In wild-type control (Ctl) mice, C5L2 mRNA expression is increased over time by 3-fold on chow diet (ANOVA p = 0.004) and by 10-fold on a diet-induced obesity diet (DIO) (ANOVA p = 0.017), with greater expression in DIO vs. chow diet (2 way-ANOVA, diet: p<0.0001) (Fig. 1A).