The overexpression of Twist in tumors of stage III–IV (56.25%) was higher than that in stage I (21.05%) and II (32.35%, P<0.01); the overexpression rate of Twist in poor differentiated cancer tissues (66.67%) was higher than that in well (20.00%) and moderate (26.79%) differentiated cancer tissues (P<0.01) and it was also higher in cases with lymphatic metastasis than in cases without lymphatic metastasis (48.58% versus 24.0%, P<0.01). The gene discussed is TWIST1; the disease is Lymphatic Metastasis.