CYP1A2 and cirrhosis of liver: Western blot analysis (a representative Western blot is shown in Fig. 4A) provided results that were fully consistent with those of qPCR determination: in liver microsomes of control rats (lanes 3–5) CYP1A1 protein was undetectable, whereas CYP1A2 was constitutively expressed at a considerable level and decreased significantly as liver cirrhosis progressed.