To gain more insight into the composition of tumor-infiltrating cells during treatment, we calculated the frequency of Foxp3+CD4+ Tregs, CD49b+CD3− NK cells and ICOS+CD4+ activated Th cells and Ki-67+CD8+ proliferating CTLs in tumor, tumor-draining lymph nodes (TDLN) and spleen on day 15 (Figure 2E–F, Figure S8). Here, ICOS is linked to neoplasm.