In order to determine the optimal treatment schedule in terms of timing of both anti-CTLA-4 and gemcitabine, we treated AB1-HA tumor-bearing mice with three different regimes: gemcitabine followed by anti-CTLA-4, concomitant combination therapy, and anti-CTLA-4 followed by gemcitabine (Figure S3; Figure 4). This evidence concerns the gene CTLA4 and neoplasm.