We then quantified the effects of preventing Ecrg4 down-regulation on mucosal thickness (Figure 5B) and cell infiltration (Figure 5C) in either (1) untreated animals (Untreated), or after (2) NTHi infection without AD (Control + NTHi), (3) NTHi infection after ADgfp pretreatment animals (ADgfp + NTHi), and (4) NTHi infection after ADEcrg4 pretreatment (ADEcrg4 + NTHi). Here, ECRG4 is linked to infection.