Whereas the infection of control or ADgfp animals resulted in a >90% decrease in mucosal Ecrg4 gene expression when compared to untreated animals (Figure 4A), Ecrg4 mRNA remained high in animals pre-injected with ADEcrg4 in spite of the NTHi infection (25 vs. 15 fold). The gene discussed is ECRG4; the disease is infection.