Fig. 2 shows 1H spectra acquired from the four preclinical experimental groups (wild type, LAL deficient, sebelipase alfa-treated LAL deficient rat liver, and a rat model of NAFLD), hepatic cholesterol and fatty acid moiety concentration measurements (as determined from the MR measurements) and a plot showing correlation between MR and biochemical assay measurements of hepatic cholesterol / cholesteryl ester content. This evidence concerns the gene LIPA and metabolic dysfunction-associated steatotic liver disease.