FUS and amyotrophic lateral sclerosis: In order to determine whether FUS-WT and ALS-linked FUS mutants undergo asymmetric dimethylation at arginine residues, we expressed FUS-WT and the FUS mutants in HEK293T cells, isolated FUS by immunoprecipitation and detected asymmetrically dimethylated arginine using an anti-asymmetric dimethylated arginine antibody (Figure 1C).