As shown in Figure 4B, bethanechol treatment also stimulated expression of Egfr and post-Egfr signaling molecules Myc and Ccnd1. Previously, in human colon cancer cells we showed that cholinergic agonist-MR interaction cross-talks with EGFR and activates post-EGFR signaling to promote cell proliferation and tumor invasion [6]. This evidence concerns the gene MYC and colonic neoplasm.