sTIE2 prevented the maturation of αSMA-positive blood vessels in HE → WT mice both at baseline and 72 h post-ligation (Fig 2C and Supporting Information Fig S2A), Likewise, the protection from ischaemic necrosis and tissue death in the crural muscle of HE → WT mice was abrogated by sTIE2 treatment (Fig 2D–G and Supporting Information Fig S2B). Here, ACTA1 is linked to hereditary elliptocytosis.