In this cohort of 123 patients, there was no evidence of a linear relationship between serum calcium and BCR (RR: 1.01, 95% CI: 0.71–1.45, P = 0.95) in multivariable analysis adjusting for pathological tumor stage, Gleason score, pre-SRT PSA, and SRT dose nor was there evidence of a difference in the risk of BCR between patients with low, moderate, and high calcium levels (RR (moderate versus low): 0.80, RR (high versus low): 1.32, and overall P = 0.21). This evidence concerns the gene BCR and neoplasm.