Manual and automated FISH results for HER2 copy number, CEP17 copy number, HER2/CEP17 ratio and the percentage of carcinoma cells with HER2/CEP17 ratio >2.2 showed high correlation (Pearson correlation coefficient 0.947, 0.705, 0.943, and 0.970, respectively). This evidence concerns the gene ERBB2 and carcinoma.