In this study, the most outstanding gene for the diagnosis of stage I NSCLC, MYF6, was found to be methylated in 65 of the 101 cases of patients with stage I NSCLC, displaying a sensitivity of 64.36%; and the methylation of MYF6 was also found in 2 of the 30 cases of patients with non-cancerous lung diseases, displaying a specificity of 93.3%. This evidence concerns the gene MYF6 and non-small cell lung carcinoma.