GZMB and melanoma: Our previous studies using B16F10 melanoma cells focused on the immune mechanisms and used Ganoderma lucidum polysaccharides to demonstrate the enhancement of MHC class I and costimulatory molecules in B16F10 cells (23), promotion of lymphocyte activation by B16F10 cells (24), antagonism against lymphocyte suppression caused by the culture super-natants of B16F10 melanoma cells (25), induction of marked cytotoxicity against B16F10 cells in cytotoxic T cells (CTLs) with granzyme B and porforin and inhibition of tumori-genesis in wild-type B16F10 melanoma cells in vivo(26).