It was shown by EGFP-annexin V/PI staining and flow cytometry that the percentage of early apoptotic cells in T-cadherin-transfected B16F10 melanoma cells was significantly larger compared with either the parental or empty pEGFP-N1-transfected B16F10 cells (P<0.05), while the percentage of early apoptotic cells in the parental and empty pEGFP-N1-transfected B16F10 cells was similar (P>0.05, Fig. 2A–D). The gene discussed is ANXA5; the disease is melanoma.