To set up an in vitro cellular model of HGPS, we generated iPSCs from two HGPS primary skin fibroblast lines (HGADFN164: HGPS-1 and HGADFN155: HGPS-2, respectively) and one age-matched normal fibroblast line (AG08470) by retroviral transduction of KLF4, SOX2, OCT4, and C-MYC cocktails [27, 28] (See table S1 for cell line information). The gene discussed is POU5F1; the disease is Hutchinson-Gilford progeria syndrome.