Hep-2/TIC group: large amount of necrotic tumor tissue, with considerable lymphocyte infiltration and rare tumor cell division; Hep-2/CD group: considerable amount of necrotic tissue, with a few infiltrating lymphocytes; Hep-2/TNF-α group: large amount of necrotic tissue, with extremely rare infiltrating lymphocytes; Hep-2/0 group: tumor tissue of lowly differentiated squamous cell carcinoma, with frequent giant cells and dividing tumor cells, no significant tumor necrosis or lymphocyte infiltration was seen. Here, TNF is linked to neoplasm.