Based on the therapeutic rationale of CD suicide gene/pro-drug system and TNF-α, theoretically they manage to treat laryngeal carcinoma via multiple pathways while showing additive effect: TNF-α induces high permeability of tumor vasculature and thereby helps gather and concentrate 5-FU, a chemotherapeutic agent converted from 5-FC, in the tumor tissue. This evidence concerns the gene TNF and neoplasm.