At day 29 after cell inoculation, volume of grafted tumor in Hep-2/TIC group, Hep-2/CD group, Hep-2/TNF-α group and Hep-2/0 group was 80.47±33.74 mm3, 135.5±14.83 mm3, 525.8±40.70 mm3 and 678.3±52.34 mm3, respectively; significant difference was seen between each two of them (P<0.05). This evidence concerns the gene TNF and neoplasm.