Hep-2/TIC group: large amount of necrotic tumor tissue, with considerable lymphocyte infiltration and rare tumor cell division; Hep-2/CD group: considerable amount of necrotic tissue, with a few infiltrating lymphocytes; Hep-2/TNF-α group: large amount of necrotic tissue, with extremely rare infiltrating lymphocytes; Hep-2/0 group: tumor tissue of lowly differentiated squamous cell carcinoma, with frequent giant cells and dividing tumor cells, no significant tumor necrosis or lymphocyte infiltration was seen. The gene discussed is TNF; the disease is squamous cell carcinoma.