In this study, to elucidate the mechanism involved in anti-tumor activities of PMS1077 (Fig. 1), we established DU145-NF-κB-Luc and PC3-NF-κB-Luc cell lines stably expressing a 4×κB promoter driving luciferase reporter and determined the effects of PMS1077 on the TNF-α induced activation of NF-κB in these cell lines. Here, NFKB1 is linked to neoplasm.