PROM1 and hepatocellular carcinoma: Interestingly, although the CD133+ and CD133− cells sorted from the SMMC-7721 and HCC-LY5 cell lines exhibited no significant differences in growth (Figure 1B), the CD133+ cells migrated and invaded to a greater extent than the CD133− cells in in vitro transwell migration and matrigel invasion assays (Figure 1C, D), indicating that CD133+ cells are highly migratory and invasive.