FN1 and Hyperglycemia: Immunoblotting of A549 cells cultured for 48 h in NG or HG conditions showed that the amount of onfFN in (Fig. 4C) and concequently total FN (Fig. 4B) in cellular extracts was increased by over-expression of GFAT, which indicates the involvement of the HBP in hyperglycemia-mediated glycosylation of fibronectin in A549 cells.