For instance, MAGE-A1 expression has been found in 48% of metastatic melanoma versus 16% of primary melanoma (30) and NY-ESO-1 has been found to be expressed in 40% of grade 3 bladder tumors and 23% of grade 2 tumors, but in none of the grade 1 tumors (31). This evidence concerns the gene MAGEA1 and urinary bladder neoplasm.