Consistently, immunohistochemistry (IHC) showed that CB-PIC treatment attenuated the expression of decreased expression of Ki-67(proliferation), CD34 (blood vessel density), and carbonic anhydrase(CA)IX (hypoxic marker) and increased expression of pAMPKα index in CB-PIC-treated group compared to untreated control, indicating that CB-PIC exerts antitumor activity via inhibition of proliferation, angiogenesis, and hypoxia along with AMPKα activation and apoptosis induction in SW620 cancer cells. This evidence concerns the gene CA9 and cancer.