We found that all of the Akt-inhibitor-sensitive cells and five out of the seven resistant cells (HCC-1937, MDA-MB-436, BT-459, HCC-1806 and JIMT-1) displayed significant Akt Thr308/Ser473 and PRAS40 Thr246 phosphorylation, confirming that the Akt signalling pathway is active in these cells. The gene discussed is AKT1; the disease is hepatocellular carcinoma.