Employing a lentiviral shRNA approach we identified five independent shRNAs that reduced the expression of SGK1 protein to near undetectable levels in the Akt-inhibitor-resistant cell lines displaying high levels of SGK1 protein (BT-549, MDA-MB-436 and JIMT-1, Figure 3A, or HCC-1937, Supplementary Figure S3 at http://www.biochemj.org/bj/452/bj4520499add.htm). The gene discussed is SGK1; the disease is hepatocellular carcinoma.