Remarkably, also two out of six low HTRA1 -expressing tumor samples derived from our breast cancer cohort showed methylation in a smaller, distal stretch of this particular region (recent data obtained by Wang et al. with the MDA-MB-468 cell line suggested that the more distal CpG sites might in fact be the most relevant ones for HTRA1 expression [15]). This evidence concerns the gene HTRA1 and breast cancer.