To test this, we examined cav-1 mRNA levels in control and IPF fibroblasts expressing wild type FoxO3a, mutant FoxO3a or GFP seeded on polymerized collagen matrices and cav-1 mRNA level was measured using quantitative RT-PCR (Fig. 3A). This evidence concerns the gene FOXO3 and idiopathic pulmonary fibrosis.