The concentration of NOx in animals with CYP-induced cystitis decreased significantly 30 minutes after adding L-NMMA (100μm) to the dialyzing aCSF; it is interesting to note that this reduction was no greater than 30% of the value of the initial samples, even after 90 minutes of perfusion with aCSF containing the NOS inhibitor, as is observed with the NOx values of the subarachnoid fluid, no greater than 30% of the value of the initial samples, even after 90min of perfusion with aCSF containing the NOS inhibitor (Figure 5). This evidence concerns the gene PPIG and chronic cystitis.