In order to determine if antagonizing NF-κB signaling inhibits PCa tumor establishment and growth in the bone microenvironment, we generated NF-κB signaling inactivated PCa cell lines by stably infecting with IKK2-KD vectors (PC3-KD and C4-2B-KD) (Fig. 2C). Here, NFKB1 is linked to neoplasm.