Western blotting analysis further confirmed that the expression of RANKL and PTHrP was increased in NF-κB activated PCa cells (LNCaP-EE), while, decreased in NF-κB inactivated PCa cells (PC3-KD), compared to empty vector control cells (LNCaP-EV and PC3-EV) (Fig. 2F). The gene discussed is NFKB1; the disease is posterior cortical atrophy.