Towards this goal, we have performed RNA-Seq on cells expressing exogenous wild-type FUS, two mutant forms of FUS (R521G, R522G) or small interfering RNA (siRNA) against FUS. The results of this study yield insights into the normal pathways influenced by wild-type FUS and how mutations lead to the pathogenesis of ALS. This evidence concerns the gene FUS and amyotrophic lateral sclerosis.