Here we investigated whether soluble factors (“migraine mediators”; [10]), i.e. CGRP (calcitonin gene related peptide), BDNF (brain derived neurotrophin factor) or TNFα (tumor necrosis factor alpha), which are relevant to triggering migraine attacks [11], [12], [13], [14], [15], [16], [17], might constitutively potentiate P2X3 receptor currents. The gene discussed is TNF; the disease is migraine disorder.