Assessment of differential protein expression between the primary tumour, VTT and the metastases revealed no significant difference between the primary tumour and VTT but significantly increased expression in the metastases compared to the primary and VTT for Ki67, p53, VEGFR1, SNAIL and SLUG (Figures 2, 3, 4, 5, 6). Here, TP53 is linked to neoplasm.