To further clarify the mechanism of MACC1 in carcinogenesis of NPC, we down-regulated MACC1 expression in EBV-negative NPC cell line CNE2 by MACC1 siRNA transfection and found that the expression of Met, p-Akt (Ser473), β-catenin and its downstream gene c-Myc were inhibited, but p-Erk1/2 expression was not affected. This evidence concerns the gene MYC and nasopharyngeal carcinoma.