To illustrate the utility of the TraceRNA algorithm for breast cancer study, we also focus on the genes interacted with the estrogen receptor alpha, ESR1, with GBmiRs including miR-18a, miR-18b, miR-193b, miR-19a, miR-19b, miR-206, miR-20b, miR-22, miR-221, miR-222, miR-29b, and miR-302c. The gene discussed is ESR1; the disease is breast cancer.