Changes in activated microglia were seen (Figure 6) using Iba1 immunoreactivity (expressed as area/mm2) which showed a significant increase in the FVLQ/+-APS group compared with the FVL adjuvant-immunized control group (Figure 6J) and the FVLQ/Q-APS group (P < 0.001). This evidence concerns the gene AIF1 and autoimmune polyendocrinopathy.