The objectives of the present study were therefore to investigate: a) the GLP-1 response to short-term overfeeding in young men; b) the difference in serum GLP-1 concentration in pre- and post- overfeeding between various adiposity groups; and c) the relationship of fasting GLP-1 with various obesity-related markers. This evidence concerns the gene GLP1R and obesity due to melanocortin 4 receptor deficiency.