We performed soft agar colony formation assays to investigate the capability of FKBP51-overexpressing melanoma cells to promote anchorage-independent growth, a typical feature of EMT.8Figure 1f shows that FKBP51 strikingly stimulated soft agar growth, as suggested by the colony number and size that were remarkably increased in FKBP51-overexpressing cells. The gene discussed is FKBP5; the disease is melanoma.