We validated this data via Western and immunofluorescence analysis of Cdkn1a and Cdkn1b (p21 and p27, respectively) in SVR angiosarcoma lines treated for 24 hours with sham or 100 μM propranolol, revealing significant upregulation of both proteins following propranolol treatment (Figure 3B & C). This evidence concerns the gene CDKN1B and angiosarcoma.