Next, we utilized Tsc2-null mouse embryonic fibroblasts and ELT3 cells (Tsc2-null cells from an Eker rat uterine leiomyoma), which are established cellular models of TSC [26], [27], [28] compared to HEK293 and A549 cells, which express TSC2. Here, TSC2 is linked to Uterine leiomyoma.