Then the MBIP technique was used to study sc, lin and competitive sc/lin DNA binding of mutp53 R273H in cell lysates (R273H stably transfected into human tumour p53-null H1299 cells; Fig. 5D, E) or endogenous R273H, R273C and G245S (glioblastoma cell lines Onda10, U251 and Onda11; not shown). This evidence concerns the gene TP53 and glioblastoma.