To explore whether chromosomal instability was an ongoing phenomenon that could generate tumour cell sub-populations with different chromosome numbers, we then created single cell clones from one MYCN amplified NB cell line (GI-MEN) and one non-amplified cell line with multiple segmental aberrations (SK-N-AS), and from hTERT transduced fibroblasts as a stable karyotype control. Here, MYCN is linked to neoplasm.