In a first step to evaluate the influence of USP7 on adenovirus infection, the generated USP7 kd cells APU6 and HU5 (79.5% and 86.6% knockdown efficiency respectively; Figure 6A and S5A) were infected with wt virus (H5pg4100), and the synthesis of early and late viral proteins, as well as the production of progeny virions were compared to those of the control cell lines at different time points (Figure 6 and S5). This evidence concerns the gene USP7 and adenoviridae infectious disease.