Since VCaP cells express high level of p536 (compared to almost undetectable signal from other PCa cells), and AR, we hypothesize that T/E fusion expressing VCaP cells would be the most sensitive PCa cell line to Celastrol treatment while LNCaP would be sensitive due to AR expression and the AR negative PC3 and DU145 cells should be relatively insensitive. This evidence concerns the gene AR and posterior cortical atrophy.