To determine expression levels and solubility of PINCH during loss of Tau solubility in disease, brain tissues from normal control, AD, HIVE and FTD patients were processed to separate proteins into different fractions based on solubility, with the RAB (RB) fraction being soluble, RIPA (RP) less soluble and the formic acid fraction (FA), insoluble (Figure 8A). This evidence concerns the gene LIMS1 and Alzheimer disease.