For each candidate GSV that was identified in at least 3 NFBC1966 subjects – the SH2B1 locus and 5 other loci (Table 1) – and also the previously-identified 16p11.2 obesity locus, we conducted a 2-way analysis of variance, with gender and GSV status as explanatory factors and log-transformed BMI as the response variable. This evidence concerns the gene SH2B1 and obesity due to melanocortin 4 receptor deficiency.