In an attempt to better understand the role of HIV-1 Tat, we construct a lentiviral vector expressing HIV-1 Tat, which could be used to infect prostate cancer lines in vitro and maintain long-term expression of HIV-1 Tat in lentivirus-infected prostate cancer lines, thus providing a foundation for the further research. The gene discussed is TAT; the disease is prostate carcinoma.