To illustrate de novo transcript assembly, PRICE was used to assembly the mRNA sequence of human keratin 6A using a published paired-end transcriptome dataset from a cutaneous squamous cell carcinomatumor of the keratoacanthoma subtype (NCBI Sequence Read Archive accession SRA029929; Figure 3) (Arron et al. 2011). The gene discussed is KRT6A; the disease is keratoacanthoma.