In this study we also observed that PIK3CA codon 545 substitutions account for 9.8% of PIK3CA mutations in CRC [49] and, since the carcinoma carrying the PIK3CA p.Glu545Asp mutation did not present mutations in either KRAS or BRAF, it is conceivable that this mutation confers some selective advantage. Here, KRAS is linked to carcinoma.