In untreated resistant tumors, Thy-1 expression levels were about 100-fold (p=0.014) higher, Oct-4 expression levels were about 14-fold increased (p=0.027), Sox-2 expression levels were 5-fold (p=0.086) upregulated and expression levels for Nanog were detected to be increased about 7-fold (p=0.05), in comparison to tumors established from passaged tumor cells (Figure 6A-D). Here, POU5F1 is linked to neoplasm.